The newly developed LaChromUltra II column is the longest UHPLC column at 250 mm in length. When used together with the reduced volume system ChromasterUltra Rs, it produces an exceptional high-separation performance.
The combination of this column and the ChromasterUltra Rs enables system pressure tolerance of 140 MPa with 50,000 or more theoretical plates per column.
A total-reflection capillary flow cell is incorporated into the diode array detector to answer to the increasing need for high-sensitivity analysis of hazardous substances, genotoxic impurities, etc. A high-sensitivity flow cell with an optical path length of 65 mm is also available. In addition to the capability of high-sensitivity analysis, carryover is reduced through the use of a double-corkscrew mixer.
The diode array detector (DAD) exhibits low noise and low drift, achieved through the use of a new optical system providing optimal conditions for high-sensitivity analysis. The optional high-sensitivity flow cell (optical path length of 65 mm) further enhances sensitivity; about a tenfold increase is obtained compared with our previous model (LaChromUltra), thus enabling high-sensitivity applications including impurities from side reactions, genotoxic impurities, etc.
The acquisition of impurity profiles during all stages of synthesis and in raw materials used in medicines and chemicals, intermediate byproducts, and finished drugs are examples of useful applications.
The binary pump is equipped with the latest design in microfluidic double-corkscrew mixers.
An efficient mixing is attained even for a low volume, resulting in a baseline that is extremely stable during gradient analysis, enabling higher-sensitivity analysis.
Structure of the double-corkscrew mixer:
Repeated branching and merging of channels within the mixer provide effective solvent mixing (mixer volume of 55 µL).
| Column | LaChromUltra II C18(1.9 µm) 2.0 mm I.D.×50 mm L. |
|---|---|
| Eluent | A)0.1% TFA/H2O(v/v) B)0.1% TFA/CH3CN(v/v) A/B=50/50 |
| Flow rate | 0.500 mL/min |
| Detection | UV 214 nm |
Low carryover (0.001% or less) is attained via the optimized injection port structure.
All modules can be controlled from the Graphical User Interface (GUI) controller.
The GUI is comprised of a color LCD monitor (5.7-inch color TFT display with LED back-light) and a touch panel made for easy viewing and simple operation.
Up to 10 programs including a timer function, pre-analysis tasks (Wakeup), and post-analysis tasks (Sleep) can be created for a system.
The GUI controller enables you to check the status of consumables usage for all units that are connected to the system.
Hitachi’ s own Moment Enhancing Mechanism (MEM) column fitting is a simple but exciting new development. The finger-tight piping integral fitting, which has a very small dead volume, is capable of safely tolerating system pressures as high as 140 MPa.
MEM column fitting
A view of the operation of attaching a column
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