Hitachi Fluorescence Spectrophotometer F-7100
Best-in-Class” Analytical Signal-to-Noise. 360 of S/N (P-P) and > 1,200 of S/N (RMS)
Increased sensitivity 1.5x compared to conventional instruments
Example of High Sensitivity Analysis (Fluorescein)
Due to its enhanced sensitivity (1.5x higher), weak signals can be detected with very low noise levels.
An example of the high sensitivity analysis for fluorescein is shown. The F-7100 detected fluorescence in the order of 1×10-13 mol/L (sub-picomol) compared with a blank sample (purified water); a useful calibration was obtained in the ultra-trace range.
Industry leading lamp lifetime
Light source with 5x*¹ longer lifetime compared to conventional instruments – lifetime of 2,500 hours*² –
Comparison of lamp lifetimes
With the application of the new Xe lamp and the improved lamp ignition power source, both luminance and the lamp lifetime were increased.
Increased lamp lifetime reduces operating Cost-of-Ownership and instrument service time.*1Comparison with the service life (recommended replacement interval) of the standard Xe lamp (PN: 650-1500) of F-7000 fluorescence spectrophotometer.*2Lifetime is usable limit. Guaranty for 500 operating hours or 6 months, whichever shorter.
Troubles due to mishandling are not within guarantee.
High-Speed Scan at 60,000 nm/min – Example of reaction tracing with a spectrum
3-D time scan spectrum of coumarin for environmental applications
The fast-speed scanning enables users to carry out measurements that have been difficult with conventional instruments. In this example, an isomerization process of coumarin in kerosene was traced by spectrum measurement at 2-second intervals and displayed as a 3-D time-scan spectrum. This is a new function in the F-7000.
Previously, a quick reaction which occurs within 1 minute could be measured only by using the fixed wavelength method. The F-7000 is capable of following such a quick reaction because of its fast scanning, measuring the entire wavelength range within 1 second.
FL intensity standardization to correct for fluorecence intensity variation over time and between instruments
Window for fluorescence intensity standardization
The variations in the fluorescence intensity over time and between instruments can be corrected. Fluorescence intensity is affected by changes in lamp brightness, room temperature, optical system, etc. The fluorescence intensity of the standard sample is measured, and the sample fluorescence intensity is converted to the fluorescence intensity relative to the standard sample. This standardization is also used for the intensity comparison between different instruments, including the analysis of humic substances in environmental water (conversion to quinine sulfate), the analysis of chlorophyll in water (conversion to fluorescein), and the specified value for reagent purity (conversion to quinine sulfate).

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