Lyncée Transmission series is powered by Digital Holographic Microscopy (DHM) for quantitative imaging and measurement of transparent and semi-transparent samples with interferometric resolution. Holograms are captured and then digitally reconstructed to extract 3D information, which is subsequently interpreted by dedicated software to provide unique and meaningful insights for applications in both life sciences and materials science.
For life sciences, DHM enables label-free imaging and provides measurements of morphology and intracellular content (dry mass). These parameters are endogenous markers of cell health status and biological processes, opening applications in cell viability, dynamics monitoring, and 4D cell tracking.
For materials science, DHM provides information on surface topography, measures thickness variation and intra-sample properties such as defect and refractive indices changes. It also tracks dynamic changes and 3D position over time. It is compatible with our stroboscopic module, enabling measurement of optical MEMS, displays, and acoustofluidic devices.
Key Features
Superior DHM Performance
- Single shot full-field imaging
- Up to 100 kfps acquisition rate
- Millisecond time resolution
Address All Experimental Needs
- Multi-objective, multi-wavelength
- Wide range of options dedicated to specific applications
- Fully upgradeable
Lyncée Transmission Series: Workflow
Data acquisition encodes the whole information about the sample in the recorded hologram in a single shot at camera rate speed. The recorded hologram is then numerically reconstructed in real time. Numerical reconstruction allows to refocus the phase image after the acquisition and to get quantitative information providing insights about the monitored sample.
Transmission DHM Models
Lyncée Transmission series (Lyncée T1, T2) offers single- and dual-laser source configurations optimized for precise optical topography. Each model is an ideal tool for its application: Lyncée T1 for most applications, and Lyncée T2 for large step height samples.
Objective Lens Sets
Objective turret supports up to six objectives from 1.25 × to 100 × magnification (air and oil immersion), which allows easy switching between wide-field overview and high-magnification inspection.
Motorized Stage
The optional motorized stage enables automated acquisitions at predefined XYZ locations to record multiple sites without user intervention. The motorized stage also allows stitching multiple acquisitions to obtain a larger field of view to investigate large samples. In life sciences it is compatible with standard multi-well microplates (up to 384 wells) for high-content and high-throughput screening assays.
Fluorescence Module
When combined with Lyncée Transmission series, the optional fluorescence module enables simultaneous quantitative phase imaging and epifluorescence observation through the same objective lens. This allows direct correlation between label-free DHM measurements and fluorescence reference assays for applications such as cardiotoxicity screening and single-cell analysis. The plug-and-play module supports multiple light sources and cameras, with dedicated filters ensuring crosstalk-free operation.
Environmental Chamber
The perfect addition for non-perturbing multi-day time-lapse experiments. It allows full control of temperature, O₂, CO₂ and humidity with the same precision as a full incubator. O₂ control (for hypoxia and hyperoxia experiments) can be added with an optional gas mixer.
Principle of Digital Holographic Microscopy
DHM Technology
For life sciences applications, Lyncée Transmission series provides real-time Quantitative Phase Imaging (QPI) of living cells. Segmentation then provides biological parameters on individual cells such as viability, confluency and cell dynamics.
